Nucleic Acid Extraction Kapa Purification Kit
Nucleic Acid Extraction Or Tlhoekiso Kitkapa e bolokoe ho -20 ℃. Mohlala o lokela ho tsamaisoa ho sebelisoa 0 ℃ curling.
Introduction
Nucleic Acid Extraction Or Purification Kit (Mokhoa oa Magnetic Beads) e etselitsoe ho itlhoekisa ka boits'oaro RNA le DNA ho tsoa marong a 'mele (joalo ka li-swabs, plasma, serum) ho sebelisa lisebelisoa tsa ho ntša li-nucleic acid. Theknoloji ea Magnetic-particle e fana ka DNA/RNA ea boleng bo holimo e loketseng ho sebelisoa ka kotloloho lits'ebetsong tse tlase tse kang amplification kapa li-enzymatic reactions tse ling.
Application Range
Madi kaofela, plasma, serum le disampole tse ding tsa dithishu di ne di ntshiwa ka ho toba le ho silwa. Nucleic acid e lokollotsoeng e ne e khabisitsoe ka mokhoa o ikhethileng ke lifaha tsa makenete tsa super paramagnetic nanometer. Joale liprotheine, li-ion tsa letsoai le inorganic letsoai le litšila tse phelang li ile tsa tlosoa ka ho hlatsoa solution.Finally, the nucleic acid has eluted with eluent to find pure nucleic acid solution.
Kit Contents
| Katse. Che. | YXN-VIRAL01-32A-BR | Likaroloana | ||
| -50A | - 100A | |||
| Boholo | 32Tes | 50Tlhahlobo | 100Tlhahlobo | |
| Lysis Buffer | 96 hantlePre-pack ed Lipoleiti 2 likotoana | 25ml | 50ml | Surfactant le Tris |
| Hlatsoa Buffer I | ★15ml | ★30ml | Tharollo e phahameng ea letsoai | |
| Hlatsoa Buffer Il | ★6ml*2 | ★12ml*2 | Tharollo e tlaase ea letsoai | |
| Elution Buffer | 10ml | 20ml | Tharollo e tlaase ea letsoai | |
| MagaBio Reagent | 1.0ml | 2.0ml | Likaroloana tsa makenete | |
| Bukana(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Nlintlha:Bakeng saYXN-VIRAL01-32A-BR-50A,eketsa 15mL Absolute ethanol ho ★15mL Hlatsoa Buffer I pele o sebelisoa; eketsa 24mL Absolute ethanol ho ★6mL Hlatsoa buffer Il pele o sebelisoa. | ||||
| Bakeng saYXN-VIRAL01-32A-BR-100A, eketsa 30mL Absolute ethanol ho ★30mL Wash Buffer I pele o sebelisoa; eketsa 48mL Absolute ethanol ho ★12mL Hlatsoa buffer Il pele u e sebelisa.【Reagents e tla lokisoa ke mosebedisi】Ka kopo itokisetse ethanol e felletseng (mophato oa tlhahlobo) u le mong. | ||||
Storage Conditons
Ha setlolo se fihla, lisebelisoa tsa kit li ka bolokoa mocheso oa kamore (15 - 25 ° C). Li-reagents li tsitsitse ho fihlela selemo se le seng ho tloha ka letsatsi la tlhahiso.
Sample Requirements
1. Sample e sebetsang: Swabs, plasma, serum le mali a felletseng joalo-joalo.
2. Mehlala ea polokelo le lipalangoang: Mohlala o lokela ho lekoa hang-hang
Materials le Devices Required but Not Provided
1. Liatlana tse se nang phofo tse lahloang
2. Setshelo sa Biohazard
3. Pensele kapa ka
Procedure
Lintlha tse latelang li sebelisa sesepa sa sesepa sa sesepa e le mohlala ho hlalosa ka bokhuts'oane mehato ea ts'ebetso ea sesebelisoa sa "nucleic acid" sa bioloji.Bioer NPA-32PkapaSMLITŠOANTŠISO 32. Bakeng sa mefuta e meng ea mehlala, ka kopo sheba buka ea mosebelisi. E ka boela ea sebetsoa ke bareki ho latela tlhahlobo ea liteko:
1. Reagent Tokisetso
a. Bakeng saYXN-VB03-32A-50A le YXN-VB03-32A-100A
Kenya 500uL Lysis Buffer kholomong ea 1 le ea 7 ea poleiti ea 2.2mL 96-deep-well, 500uL Wash Buffer I kholomong ea 2 le 8, 500uL Wash Buffer II kholomong ea 3, 4 le 9,10; 70uL Elution Buffer ho kholomo ea 5 le 11, 180uL ea metsi a hloekileng le 20uL MagaBio Reagent ho kholomo ea 6 le 12 (lifaha tsa makenete li lokela ho tsoakoa ka botlalo pele li sebelisoa),
b. Bakeng saYXN-VB03-32A
Beha li-reagents tse 96 tse pakiloeng hantle ka mocheso oa kamore. Hlohlelletsa poleiti e nang le liliba tse 96 ka holimo ka makhetlo a mararo, 'me u tabole mokotla oa polasetiki. Centrifuge reagent e kentsoeng pele ka metsotsoana e seng mekae (kapa tsokotsa ka letsoho ka makhetlo a 'maloa) ho qoba reagent e khomarela lebota la li-tubes. Haha filimi ea foil ea aluminium ea poleiti ea liliba tse 96 'me u khetholle moo poleiti e eang teng (lifaha tsa makenete kholomong ea #6 le #12),
2.Mohlala Ho ntsha
1. Eketsa sampole ea 300uL ho likholomo tse 96 tsa lipoleiti tsa seliba #1 le #7, ka kopo qoba tšilafalo,
2. Beha sejana se tebileng sa 96 sesebelisoa, kenya malebela a 8-strip ho sesebelisoa,
3. Tsamaisa lenaneo ho latela mekhoa e latelang,
4. Ka mor'a hore ho itlhoekisa othomathike ho felile, fetisetsa elution Buffer ka litšiea 5 le 11 ho e hloekileng antinuclear 0.5mL centrifuge tube; haeba u sa e sebelise hang-hang, ka kopo boloka ho -20 ° C.
Performance Characteristics
1. Sehlahisoa se ntšitsoeng se fumanoa ka mokhoa o phahameng oa kutloisiso ea HBV DNA reagent ho fihlela kutloisiso ea 10 IU / mL. Sehlahisoa se ntšitsoeng se bonoa ke setifikeiti sa ho lemoha se phahameng sa HCV RNA ho fihlela kutloisiso ea 50 IU/mL.
2. Khetha li-samples tsa 4 (serum / plasma sample, nasopharyngeal swab sample, cervical exfoliated cell sample), e 'ngoe le e' ngoe e hlapolotsoe ka makhetlo a 10 ka li-gradients tsa 3 (ho kenyeletsoa le sampuli ea pele ea likarolo tsa 4), ho sebelisa li-reagents tse tšoanelehang le li-agent tsa liteko ho lemoha ka hare. gene ea referense ho latela litaelo tsa sehlahisoa, 'me boleng ba Ct ba batch ka' ngoe bo fapana ka tlase ho 1.
| Step | Hantle Sebaka | Program Lebitso | Waitng Time(min:SS) | Mixng Time(mho:SS) | Magnet Time(min:SS) | Adsorption | Speed | Volume lintlha(μL) | TEmperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Beads | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wmolora1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wmolora2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wmolora3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Lahla | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GENESEAL POLOKO.
Ho sebelisa sehlahisoa sena ka tsela e sa hlalosoang litokomaneng tsa mosebelisi ho ka baka kotsi kapa tšenyo ea sesebelisoa kapa sesebelisoa. Netefatsa hore mang kapa mang ea sebelisang sehlahisoa sena o fumane litaelo ka mekhoa e akaretsang ea ts'ireletso bakeng sa lilaboratori le lintlha tsa polokeho tse fanoeng tokomaneng ena.
1.1 Pele o sebelisa sesebelisoa kapa sesebelisoa, bala le ho utloisisa lintlha tsa polokeho tse fanoeng litokomaneng tsa mosebelisi tse fanoeng ke moetsi oa sesebelisoa kapa sesebelisoa.
1.2 Pele u sebetsana le lik'hemik'hale, bala 'me u utloisise lintlha tsohle tse sebetsang tsa Safety Data Sheets (SDSs) 'me u sebelise lisebelisoa tse loketseng tsa ho itšireletsa (li-gloves, mese, tšireletso ea mahlo, joalo-joalo). Ho fumana li-SDS, bona karolo ea "Documentation and Support" tokomaneng ena.
2. Lik'hemik'hale polokeho
KAKARETSO TŠOARO EA K'hemik'hale. Ho fokotsa likotsi, etsa bonnete ba hore basebetsi ba laboratori ba bala le ho sebelisa litataiso tse akaretsang tsa polokeho bakeng sa tšebeliso ea lik'hemik'hale, polokelo, le litšila tse fanoeng ka tlase, 'me u buisane le SDS e amehang bakeng sa litemoso le litaelo tse khethehileng: Bala le ho utloisisa Safety Data Sheets (SDSs) e fanoeng ke k'hemik'hale. moetsi pele o boloka, o tšoara, kapa o sebetsa ka lik'hemik'hale life kapa life kapa lisebelisoa tse kotsi.
2.1 Fokotsa ho kopana le lik'hemik'hale. Apara lisebelisoa tse loketseng tsa ho itšireletsa ha u tšoara lik'hemik'hale (mohlala, likhalase tsa tšireletso, liatlana kapa liaparo tse itšireletsang).
2.2 Fokotsa ho hema ha lik'hemik'hale. Se ke oa siea lijana tsa lik'hemik'hale li butsoe. Sebelisa feela ka moea o lekaneng (mohlala, fume hood).
2.3 Lekola khafetsa hore na ha ho na ho lutla kapa ho qhalla ha lik'hemik'hale. Haeba ho na le ho lutla kapa ho qhalana, latela mekhoa ea ho hloekisa ea moetsi joalokaha e khothalelitsoe ho SDS.
2.4 Tšoara litšila tsa lik'hemik'hale ka sekoting sa mosi.
2.5 Netefatsa ts'ebeliso ea linkho tsa pele le tsa bobeli tsa litšila. (Tšenyo ea mantlha
setshelo se ts'oara litšila hanghang. Sets'oants'o sa bobeli se na le marotholi kapa ho lutla ho tsoa setšelong sa mantlha. Lits'oants'o ka bobeli li tlameha ho tsamaellana le litšila 'me li finyelle litlhoko tsa mmuso, tsa naha le tsa lehae bakeng sa polokelo ea lijana.).
2.6 Ka mor'a ho tšolla sets'oants'o sa litšila, se tiise ka kepisi e fanoeng.
2.7 Hlalosa (ka tlhahlobo ha ho hlokahala) litšila tse hlahisoang ke lisebelisoa tse itseng, li-reagents, le li-substrates tse sebelisoang laboratoring ea hau.
2.8 Netefatsa hore litšila lia bolokoa, tsa fetisoa, tsa tsamaisoa, le ho lahloa ho latela melaoana eohle ea lehae, ea naha, ea provense le/kapa ea naha.
2.9 Lisebelisoa tse nang le mahlaseli a kotsi kapa biohazard li ka hloka ho tšoaroa ka mokhoa o khethehileng, 'me ho ka ba le meeli ea ho lahla.
3.Biological kotsi polokeho
Biohazard e ka bang teng. Ho itšetlehile ka mehlala e sebelisoang sesebelisoa sena, bokaholimo bo ka nkoa e le biohazard. Sebelisa mekhoa e nepahetseng ea ho tlosa litšila ha u sebetsa le likokoana-hloko.
MOHLOMI. Lisampole tsa baeloji tse kang lisele, maro a 'mele, lintho tse tšoaetsanoang, le mali a batho le a liphoofolo tse ling li na le monyetla oa ho fetisetsa mafu a tšoaetsanoang. Latela melaoana eohle e sebetsang ea lehae, ea setereke / ea provense, le/kapa naha. Apara lisebelisoa tse nepahetseng tsa ts'ireletso, tse kenyelletsang empa li sa felle feela ho: liaparo tse sireletsang mahlo, thebe ea sefahleho, seaparo / jase ea lab, le liatlana. Mosebetsi oohle o lokela ho etsoa litsing tse nang le thepa e nepahetseng ho sebelisoa lisebelisoa tse nepahetseng tsa polokeho (mohlala, lisebelisoa tsa 'mele). Batho ba lokela ho koetlisoa ho latela melao e sebetsang le litlhoko tsa k'hamphani/setheo pele ba sebetsa ka thepa e ka tšoaetsanoang.
Bala le ho latela litataiso tse sebetsang le/kapa litlhoko tsa taolo ho tse latelang:
Naheng ea Amerika: Litataiso tsa Lefapha la Bophelo bo Botle le Litšebeletso tsa Botho la US tse phatlalalitsoeng ho Biosafety in Microbiological and Biomedical Laboratories e fumanoang ho: www.cdc.gov/biosafety.
Maemo a Tšireletseho le Bophelo bo Botle mosebetsing, Likokoana-hloko tsa Bloodborne (29 CFR§1910.1030), li fumanoa ho:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Liprothokholo tsa k'hamphani ea hau/setheo sa Biosafety Program bakeng sa ho sebetsa ka/ho sebetsana le thepa e ka bang teng ea tšoaetso. Lintlha tse ling mabapi le tataiso ea biohazard li fumaneha ho: www.cdc.gov.
Ho EU: Sheba litataiso le melao ea lehae mabapi le tšireletso ea biohazard le biosafety precaution 'me u bue ka mekhoa e metle e phatlalalitsoeng ho World Health Organisation (WHO) Laboratory Biosafety Manual, khatiso ea boraro, e fumanoang ho: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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